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Applied and Environmental Microbiology, November 2009, p. 7280-7282, Vol. 75, No. 22
0099-2240/09/$08.00+0     doi:10.1128/AEM.01870-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Cadherin Fragments from Anopheles gambiae Synergize Bacillus thuringiensis Cry4Ba's Toxicity against Aedes aegypti Larvae {triangledown}

Youngjin Park,1 Gang Hua,1 Mohd Amir F. Abdullah,3 Khalidur Rahman,1 and Michael J. Adang1,2*

Departments of Entomology,1 Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602-2603,2 InsectiGen, Inc., 425 River Road, Athens, Georgia 30602-27713

Received 4 August 2009/ Accepted 22 September 2009

A peptide from cadherin AgCad1 of Anopheles gambiae larvae was reported as a synergist of Bacillus thuringiensis subsp. israelensis Cry4Ba's toxicity to the Anopheles mosquito (G. Hua, R. Zhang, M. A. Abdullah, and M. J. Adang, Biochemistry 47:5101-5110, 2008). We report that CR11 to the membrane proximal extracellular domain (MPED) (CR11-MPED) and a longer peptide, CR9 to CR11 (CR9-11), from AgCad1 act as synergists of Cry4Ba's toxicity to Aedes aegypti larvae, but a Diabrotica virgifera virgifera cadherin-based synergist of Cry3 (Y. Park, M. A. F. Abdullah, M. D. Taylor, K. Rahman, and M. J. Adang, Appl. Environ. Microbiol. 75:3086-3092, 2009) did not affect Cry4Ba's toxicity. Peptides CR9-11 and CR11-MPED bound Cry4Ba with high affinity (13 nM and 23 nM, respectively) and inhibited Cry4Ba binding to the larval A. aegypti brush border membrane. The longer CR9-11 fragment was more potent than CR11-MPED in enhancing Cry4Ba against A. aegypti.

* Corresponding author. Mailing address: Department of Entomology, University of Georgia, Athens, GA 30602-2603. Phone: (706) 542-2436. Fax: (706) 542-2279. E-mail: adang{at}uga.edu

{triangledown} Published ahead of print on 2 October 2009.

Applied and Environmental Microbiology, November 2009, p. 7280-7282, Vol. 75, No. 22
0099-2240/09/$08.00+0     doi:10.1128/AEM.01870-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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