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Applied and Environmental Microbiology, July 2005, p. 3633-3641, Vol. 71, No. 7
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.7.3633-3641.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Purification and Characterization of a Novel Alcohol Dehydrogenase from Leifsonia sp. Strain S749: a Promising Biocatalyst for an Asymmetric Hydrogen Transfer Bioreduction

Kousuke Inoue, Yoshihide Makino, and Nobuya Itoh*

Biotechnology Research Center, Toyama Prefectural University, Kosugi, Toyama, Japan

Received 7 October 2004/ Accepted 30 January 2005

To find microorganisms that could reduce phenyl trifluoromethyl ketone (PTK) to (S)-1-phenyltrifluoroethanol [(S)-PTE], styrene-assimilating bacteria (ca. 900 strains) isolated from soil samples were screened. We found that Leifsonia sp. strain S749 was the most suitable strain for the conversion of PTK to (S)-PTE in the presence of 2-propanol as a hydrogen donor. The enzyme corresponding to the reaction was purified homogeneity, characterized and designated Leifsonia alcohol dehydrogenase (LSADH). The purified enzyme had a molecular weight of 110,000 and was composed of four identical subunits (molecular weight, 26,000). LSADH required NADH as a cofactor, showed little activity with NADPH, and reduced a wide variety of aldehydes and ketones. LSADH catalyzed the enantioselective reduction of some ketones with high enantiomeric excesses (e.e.): PTK to (S)-PTE (>99% e.e.), acetophenone to (R)-1-phenylethanol (99% e.e.), and 2-heptanone to (R)-2-heptanol (>99% e.e.) in the presence of 2-propanol without an additional NADH regeneration system. Therefore, it would be a useful biocatalyst.

* Corresponding author. Mailing address: Biotechnology Research Center, Toyama Prefectural University, Kosugi, Toyama 939-0398, Japan. Phone: 81-766-56-7500, ext. 560. Fax: 81-766-56-2498. E-mail: itoh{at}pu-toyama.ac.jp.

Applied and Environmental Microbiology, July 2005, p. 3633-3641, Vol. 71, No. 7
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.7.3633-3641.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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