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Appl Environ Microbiol, January 1998, p. 221-225, Vol. 64, No. 1
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The TRI11 Gene of Fusarium sporotrichioides Encodes a Cytochrome P-450 Monooxygenase Required for C-15 Hydroxylation in Trichothecene Biosynthesis

Nancy J. Alexander, Thomas M. Hohn,* and Susan P. McCormick

Mycotoxin Research Unit, National Center for Agricultural Utilization Research, U.S. Department of Agriculture/Agricultural Research Service, Peoria, Illinois 61604

Received 12 June 1997/Accepted 27 October 1997

Several genes in the trichothecene biosynthetic pathway of Fusarium sporotrichioides have been shown to reside in a gene cluster. Sequence analysis of a cloned DNA fragment located 3.8 kb downstream from TRI5 has led to the identification of the TRI11 gene. The nucleotide sequence of TRI11 predicts a polypeptide of 492 residues (Mr = 55,579) with significant similarity to members of the cytochrome P-450 superfamily. TRI11 is most similar to several fungal cytochromes P-450 (23 to 27% identity) but is sufficiently distinct to define a new cytochrome P-450 gene family, designated CYP65A1. Disruption of TRI11 results in an altered trichothecene production phenotype characterized by the accumulation of isotrichodermin, a trichothecene pathway intermediate. The evidence suggests that TRI11 encodes a C-15 hydroxylase involved in trichothecene biosynthesis.

* Corresponding author. Mailing address: National Center for Agricultural Utilization Research, U.S. Department of Agriculture/Agricultural Research Service, 1815 N. University St., Peoria, IL 61604. Phone: (309) 681-6380. Fax: (309) 681-6665. E-mail: thohn{at}mail.ncaur.usda.gov.




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