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Appl Environ Microbiol. 1985 August; 50(2): 503-507

Production of cloned human leukocyte interferon by Bacillus subtilis: optimal production is connected with restrained growth.

H P Meyer and A Fiechter

ABSTRACT

Bacillus subtilis, transformed with a plasmid containing the human alpha-2 (leukocyte) interferon gene, was cultivated in batch and continuous culture in a complex medium. In continuous culture with dissolved oxygen of less than 10% of air saturation, the extracellular interferon titer decreased sharply when the growth rate was lower or higher than the optimal one (mu = 0.14 h-1). Thus, a relatively low growth rate was best for extracellular interferon production, and oxygen limitation enhanced interferon production. The mean output rate in batch culture after successful harvest was 20 X 10(6) IU/liter per h and the maximal output rate in continuous culture was 14 X 10(6) IU/liter per h.

Appl Environ Microbiol. 1985 August; 50(2): 503-507






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