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FIG. 1. The recombinant model of S. cerevisiae expresses the plant cytochrome P450 73A1 and catalyzes the bioconversion of trans-cinnamic acid into p-coumaric acid (CA4H activity). (a) Schematic representation of the P450 73A1 and its yeast-associated proteins, the NADPH CPR, the cytochrome (Cyt.) b₅, and the NADH cytochrome b₅ reductase bound to the endoplasmic reticulum (ER) membrane. (b) Genetic construction of the recombinant model of S. cerevisiae. The YeDP60/CA4H plasmid was used to transform the S. cerevisiae W303-1B strain overproducing yeast CPR [W(R) strain]. ORF, open reading frame; PGK, phosphoglycerate kinase; ter, terminator; ori, origin of replication.

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