AEM Accepts, published online ahead of print on 23 October 2009

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Appl. Environ. Microbiol. doi:10.1128/AEM.01648-09
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Vegetation and soil environment influence the spatial distribution of root-associated fungi in a mature beech-maple forest.

David J. Burke^*, Juan C. López-Gutiérrez, Kurt A. Smemo, and Charlotte R. Chan

The Holden Arboretum, 9500 Sperry Road, Kirtland, OH, USA; The Department of Biology, Case Western Reserve University, Cleveland, OH 44106, USA

^* To whom correspondence should be addressed. Email: david.j.burke{at}case.edu.

Although the diversity of root-associated fungi can be quite high, the effect of plant distribution and soil environment on root-associated fungal communities at fine spatial scales has received little attention. Here, we examine how soil environment and plant distribution affect the occurrence, diversity and community structure of root-associated fungi at local patch scales within a mature forest. We used terminal restriction fragment length polymorphism (TRFLP) and sequence analysis to detect 63 fungal species representing 28 different genera colonizing tree root tips. At least 32 species matched previously identified mycorrhizal fungi, with the remaining fungi including both saprotrophic and parasitic species. Root fungal communities were significantly different between June and September, suggesting a rapid temporal change in root fungal communities. Plant distribution affected root fungal communities, with some root fungi positively correlated with tree diameter and herbaceous plant coverage. Some aspects of the soil environment were correlated with root fungal community structure, with the abundance of some root fungi positively correlated with soil pH and moisture content in June, and with soil phosphorous (P) in September. Fungal distribution and community structure may be governed by plant-soil interactions at fine spatial scales within a mature forest. Soil P may play a role in structuring root fungal communities at certain times of the year.