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AEM Accepts, published online ahead of print on 18 July 2008
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Appl. Environ. Microbiol. doi:10.1128/AEM.01099-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

The amino acid substitution at position 740 in {sigma}70 of Ralstonia solanacearum strain OE1-1 affects its in planta growth

Ayami Kanda, Kazuhiro Tsuneishi, Ai Mori, Kouhei Ohnishi, Akinori Kiba, and Yasufumi Hikichi*

Laboratory of Plant Pathology & Biotechnology, Kochi University, Nankoku, Kochi, 783-8502, Japan; Research Institute of Molecular Genetics, Kochi University, Nankoku, Kochi 783-8502, Japan

* To whom correspondence should be addressed. Email: yhikichi{at}cc.kochi-u.ac.jp.


   Abstract

Growth of Ralstonia solanacearum strain OE1-1 (OE1-1) in roots after invasion is required for its virulence. The Arg740Cys substitution in {sigma}70 of OE1-1 resulted in its loss of in planta growth and virulence. The negative dominance of mutant {sigma}70 over the wild-type protein suggested that the amino acid substitution may affect in planta growth of OE1-1, leading to lacking its virulence.







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