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Appl. Environ. Microbiol. doi:10.1128/AEM.00303-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Selective Isolation and Rapid Identification of Members of the Genus Micromonospora

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Graduate University of Chinese Academy of Sciences, Beijing 100049, China

^* To whom correspondence should be addressed. Email: huangy{at}im.ac.cn.

	Abstract

Improved methods for selective isolation of diverse actinomycetes of the genus Micromonospora, and a genus-specific nested PCR for rapid identification of putative Micromonospora isolates were developed. The robustness of both the isolation and identification approaches was underpinned by phylogenetic analysis based on 16S rRNA gene sequences.