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Applied and Environmental Microbiology, July 2008, p. 4359-4365, Vol. 74, No. 14
0099-2240/08/$08.00+0     doi:10.1128/AEM.02499-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Novel Bacterial Surface Display Systems Based on Outer Membrane Anchoring Elements from the Marine Bacterium Vibrio anguillarum{triangledown} ,{dagger}

Zhao Yang, Qin Liu,* Qiyao Wang, and Yuanxing Zhang*

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China

Received 6 November 2007/ Accepted 6 May 2008

Surface display of heterologous peptides and proteins such as receptors, antigens, and enzymes on live bacterial cells is of considerable value for various biotechnological and industrial applications. In this study, a series of novel cell surface display systems were examined by using Vibrio anguillarum outer membrane protein and outer membrane lipoprotein as anchoring motifs. These display systems consist of (i) the signal sequence and first 11 N-terminal amino acids of V. anguillarum outer membrane lipoprotein Wza, or the signal sequence and first 9 N-terminal amino acids of the mature major Escherichia coli lipoprotein Lpp, and (ii) transmembrane domains of V. anguillarum outer membrane proteins Omporf1, OmpU, or Omp26La. In order to assay the translocation efficiency of constructed display systems in bacteria, green fluorescent protein (GFP) was inserted to the systems and the results of GFP surface localization confirmed that four of the six surface display systems could successfully display GFP on the E. coli surface. For assaying its potential application in live bacteria carrier vaccines, an excellent display system Wza-Omporf1 was fused with the major capsid protein (MCP) of large yellow croaker iridovirus and introduced into attenuated V. anguillarum strain MVAV6203, and subsequent analysis of MCP surface localization proved that the novel display system Wza-Omporf1 could function as a strong tool in V. anguillarum carrier vaccine development.

* Corresponding author. Mailing address: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. Phone: 86 21 64253065. Fax: 86 21 64253025. E-mail for Q. Liu: qinliu{at}ecust.edu.cn. E-mail for Y. Zhang: yxzhang{at}ecust.edu.cn

{triangledown} Published ahead of print on 16 May 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, July 2008, p. 4359-4365, Vol. 74, No. 14
0099-2240/08/$08.00+0     doi:10.1128/AEM.02499-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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