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Applied and Environmental Microbiology, September 2005, p. 5572-5576, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5572-5576.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Genome-Wide Transcriptional Variation within and between Steady States for Continuous Growth of the Hyperthermophile Thermotoga Maritima

Keith R. Shockley,1,{dagger} Kevin L. Scott,2 Marybeth A. Pysz,1,{ddagger} Shannon B. Conners,1 Matthew R. Johnson,1 Clemente I. Montero,1 Russell D. Wolfinger,2 and Robert M. Kelly1*

Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27695-7905,1 SAS Institute Inc., 100 SAS Campus Drive, Cary, North Carolina 275132

Received 28 December 2004/ Accepted 4 April 2005

Maltose-limited, continuous growth of the hyperthermophile Thermotoga maritima at different temperatures and dilution rates (80°C/0.25 h–1, 80°C/0.17 h–1, and 85°C/0.25 h–1) showed that transcriptome-wide variation in gene expression within mechanical steady states was minimal compared to that between steady states, supporting the efficacy of chemostat-based approaches for functional genomics studies.

* Corresponding author. Mailing address: Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905. Phone: (919) 515-6396. Fax: (919) 515-3465. E-mail: rmkelly{at}eos.ncsu.edu.

{dagger} Present address: The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609.

{ddagger} Present address: Roswell Park Cancer Institute, Department of Pharmacology and Therapeutics, Elm and Carlton Streets, Buffalo, NY 14263.

Applied and Environmental Microbiology, September 2005, p. 5572-5576, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5572-5576.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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